Cell Loss from Human Gastric Mucosa Measured by the Estimation of Deoxyribonucleic Acid (dna) in Gastric Washings
نویسندگان
چکیده
D. N. CROFT, D. J. POLLOCK, AND N. F. COGHILL (London) Using a method of saline perfusion the rate of accumulation of DNA in the stomach was measured in patients with normal gastric mucosa, with pernicious anaemia, and with simple atrophic gastritis. Patients with normal mucosa, or pernicious anaemia, had low DNA rates. A majority of patients with simple atrophic gastritis had much higher values. In gastric biopsy specimens significantly higher epithelial mitosis counts were found in treated pernicious anaemia and simple atrophic gastritis than in normal mucosa. The data appear to indicate a higher turnover of surface epithelial cells in atrophic than in normal gastric mucosa.
منابع مشابه
Cell loss from human gastric mucosa measured by the estimation of deoxyribonucleic acid (DNA) in gastric washings.
D. N. CROFT, D. J. POLLOCK, AND N. F. COGHILL (London) Using a method of saline perfusion the rate of accumulation of DNA in the stomach was measured in patients with normal gastric mucosa, with pernicious anaemia, and with simple atrophic gastritis. Patients with normal mucosa, or pernicious anaemia, had low DNA rates. A majority of patients with simple atrophic gastritis had much higher value...
متن کاملProtein loss and cell loss from the small-intestinal mucosa.
Total protein and deoxyribonucleic acid (dna) were measured in small-intestinal washings from rats with normal and high rates of dna (or cell) loss. There was a significant relationship between the loss of these two substances. Preparations of isolated viable epithelial cells contained much less protein in relation to dna than did the intestinal washings. It was calculated that only 8 to 15% of...
متن کاملSodium hydrosulfide upregulates mRNA and protein expression of TGF-α in gastric mucosa in experimental model of stimulated gastric acid secretion in rats
Objective(s): Transforming growth factor alpha (TGF-α) has been shown to modulate the gastric acid secretion. Therefore, the aim of the present study was to investigate the effect of sodium hydrosulfide (NaHS) on TGF-α expression in gastric mucosa in rats. Materials and Methods: Eighteen rats were randomly divided into 3 groups (6 per group). To determine the effect of NaHS on gene and protein ...
متن کاملProtein loss and cell loss from the small - intestinal mucosal
Total protein and deoxyribonucleic acid (DNA) were measured in small-intestinal washings from rats with normal and high rates of DNA (or cell) loss. There was a significant relationship between the loss of these two substances. Preparations of isolated viable epithelial cells contained much less protein in relation to DNA than did the intestinal washings. It was calculated that only 8 to 15% of...
متن کاملEffect of Transforming Growth Factor-β (TGF-β) on proliferation of gastric epithelial cells in culture
Objective: Helicobacter pylori has a well-established role in the development of gastric cancer. In vitro studies reveal increased proliferation of the gastric mucosa in the presence of H. pylori infection. It has been also shown that production of some cytokines, such as interleukin-1 beta (IL-1b) is in...
متن کامل